| Shu, X., Shi, Y., Huang, Y. et al. Transcription tuned by S-nitrosylation underlies a mechanism for Staphylococcus aureus to circumvent vancomycin killing. Nature Communications 14, 2318 (2023). |
| Journal |
Nature Communications |
| doi |
10.1038/s41467-023-37949-0 |
IF(2022) |
16.6 |
| Services |
Polyclonal Antibody Production |
System |
Antibody Development |
| Five microliters of the diluted samples were saved as input sample at −20 °C, and another 500 μL was incubated with 2 μg of anit-MgrA polyclonal antibody (commercially prepared by MerryBio) or anit-WalR polyclonal antibodyor the corresponding IgG antibodyovernight at 4 °C with gentle rotation. |
| Zou H, Ben T, Wu P, et al. Effective anti-inflammatory phenolic compounds from dandelion: identification and mechanistic insights using UHPLC-ESI-MS/MS, fluorescence quenching and anisotropy, molecular docking and dynamics simulation. Food Science and Human Wellness, 2023, 12(6): 2184-2194. |
| Journal |
Food Science and Human Wellness |
| doi |
10.1016/j.fshw.2023.03.031 |
IF(2022) |
7.0 |
| Services |
Plasmid construction & Protein purification |
The coding regions of human MD-2 (1-149) (Gene ID: 23643) and TLR4 (27-631) (Gene ID: 7099) were codon optimized, synthesized, and inserted into the restriction sites, NdeI and HindIII of the pET30a(+) expression vector (MerryBio Co., Ltd).
The final constructs containing a His6-tag at the N-termini of MD-2 and TLR4 were verified by DNA sequencing (MerryBio Co., Ltd.).
The obtained protein was then purified by metal ion affinity chromatography using a Ni-IDA column (45–165 µm) (MerryBio Co., Ltd.) equilibrated with the lysis buffer, washed with a buffer solution (50 mmol/L Tris-HCl, 300 mmol/L NaCl, 8 mol/L Urea, 20 mmol/L imidazole) and eluted with another buffer solution (50 mmol/L Tris-HCl, 300 mmol/L NaCl, 8 mol/L Urea, 500 mmol/L imidazole).
|
| Yu Q, Li C, Niu Q, et al. Hepatic COX1 loss leads to impaired autophagic flux and exacerbates nonalcoholic steatohepatitis. Acta Pharmaceutica Sinica B. 2023;13(6):2628-2644. |
| Journal |
Acta Pharmaceutica Sinica B |
| doi |
10.1016/j.apsb.2023.03.008 |
IF(2022) |
14.5 |
| Services |
Recombinant Protein Production |
System |
Prokaryotic Expression |
In brief, recombinant mouse COX1 protein (Lot No. P04012021; MerryBio, Nanjing, China) was immobilized via amine coupling on a flow cell of the chip.
Recombinant mouse WIPI2 (Lot No. P30093114; MerryBio, Nanjing, China) was diluted in the running buffer and then injected at different concentrations and passed over adjacent target and control flow cells at a flow rate of 30 μL/min for 180 s, and the decomposition period was adjusted to 300 s.
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